微环境双重响应壳聚糖基因载体及其制备方法和应用

Microenvironment double-response chitosan gene carrier as well as preparation method and application thereof

Abstract

本发明提供了一种微环境双重响应壳聚糖基因载体及其制备方法和应用,该方法从壳聚糖低聚寡糖出发,合成了含酰腙键和二硫键的大分子壳聚糖(SRCS)。采用共价接枝方法对SRCS进行聚乙烯亚胺和聚乙二醇接枝,得到氧化还原微环境和酸微环境双重响应聚合物SRCS‑g‑PEI‑g‑PEG。本发明所制备的SRCS‑g‑PEI‑g‑PEG聚合物具有对微环境双重响应控制释放基因的性能。本发明还提供该方法获得的SRCS‑g‑PEI‑g‑PEG聚合物作为基因载体在基因转染中的应用,表现出良好的生物相容性和高的基因转染效率,是基因转染的优良载体,具有广阔的应用前景。
The invention provides a microenvironment double-response chitosan gene carrier as well as a preparation method and application thereof. The method is started from oligosaccharides of chitosan, and synthesizes macromolecular chitosan (SRCS) containing an acylhydrazone bond and a disulfide bond. The method adopts a covalent grafting method to carry out polyethyleneimine (PEI) grafting and polyethylene glycol (PEG) grafting on the SRCS, thus obtaining oxidation reduction microenvironment and acid microenvironment double-response SRCS-g-PEI-g-PEG polymer. The SRCS-g-PEI-g-PEG polymer prepared by the invention has performance of microenvironment double-response gene release control. The invention also provides the application of the SRCS-g-PEI-g-PEG polymer obtained by the method as a gene carrier in gene transfection; the SRCS-g-PEI-g-PEG polymer has good biocompatibility and high gene transfection efficiency, thus being a good carrier for gene transfection and having a wide application prospect.

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