一种四倍体马铃薯高通量测序开发标记的方法及其应用

Method for developing marker through tetraploid potato high-throughput sequencing and application of method

Abstract

The invention discloses a method for developing a marker through tetraploid potato high-throughput sequencing and an application of the method. The method for developing the marker through the tetraploid potato high-throughput sequencing disclosed by the invention can greatly reduce the complexity of a potato genome and can facilitate operation; and by virtue of the method, high-density SNP (single nucleotide polymorphism) sites can be rapidly identified, a chromosome label difference genetic interval for controlling target character can be obtained, and finally, the molecular marker can be developed on the basis of the difference genetic interval. With the application of the method disclosed by the invention, the application of an 2b-RAD sequencing technology in potato tetraploid material marker development is successfully achieved; the difficulty on developing the potato tetraploid material marker is solved, and meanwhile, a development cycle of the character closely linked marker is greatly shortened. In addition, the method disclosed by the invention also provides a reference for genetic interval mining, marker development and genome research of other tetraploid organisms having complex genomes.
本发明公开了一种四倍体马铃薯高通量测序开发标记的方法及其应用。本发明的四倍体马铃薯高通量测序开发标记的方法大幅降低马铃薯基因组复杂度,操作简便,可快速鉴定出高密度的SNP位点,获得控制目标性状的染色体标签差异遗传区间,最后基于差异遗传区段可开发分子标记。本发明的方法成功的实现了2b‑RAD测序技术在马铃薯四倍体材料标记开发中的应用,不仅解决了马铃薯四倍体材料标记开发困难的问题,并且极大缩短了与性状紧密连锁标记的开发周期。此外,本发明的方法也为其它多倍体复杂基因组生物的遗传区段挖掘、标记开发和基因组研究提供参考。

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